DNA Extraction

 

Extracting DNA! In this lesson students learned all about deoxyribonucleic acid, more commonly known as DNA. After getting a brief overview of DNA’s role in the storage of genetic information, students followed a DNA extraction protocol experiment and successfully extracted DNA from strawberries/bananas by breaking down the cell membranes with detergent, then freeing the DNA from its protective proteins (histones) by using a protease (an enzyme that breaks down protein – actually Accent Meat Tenderizer!). By breaking down the cell wall, membrane, and nuclear membrane, students were able to isolate the nucleic acids. They then added some very cold isopropyl alcohol to the resulting lysate (the fluid and cell remnants left over when the cells were broken up), which caused the DNA to become visible at the border between the aqueous solution and the alcohol. The addition of alcohol to the cell lysate allows the nucleic acids become visible without a microscope! The activity involved a number of different steps and helped emphasize the importance of following directions carefully. The young scientists were rewarded for their diligence by seeing DNA in their test cups at the end of the lab! Try building “edible DNA” at home: https://www.wikihow.com/Make-an-Edible-DNA-Model

Extrayendo ADN! En esta lección, los estudiantes aprendieron todo sobre el ácido desoxirribonucleico, más conocido como ADN. Después de obtener una breve descripción del papel del ADN en el almacenamiento de información genética, los estudiantes siguieron un experimento de protocolo de extracción de ADN y extrajeron con éxito el ADN de las fresas / bananos rompiendo las membranas celulares con detergente y luego liberando el ADN de sus proteínas protectoras (histonas). mediante el uso de una proteasa (una enzima que descompone la proteína, ¡en realidad el Acent Bee Tenderizer! Al romper la pared celular, la membrana y la membrana nuclear, los estudiantes pudieron aislar los ácidos nucleicos. Luego agregaron algo de alcohol isopropílico muy frío al lisado resultante (el líquido y los restos de células que quedaron cuando las células se rompieron), lo que hizo que el ADN se hiciera visible en el límite entre la solución acuosa y el alcohol. La adición de alcohol al lisado celular permite que los ácidos nucleicos se vuelvan visibles sin un microscopio. La actividad involucró varios pasos diferentes y ayudó a enfatizar la importancia de seguir las instrucciones cuidadosamente. ¡Los jóvenes científicos fueron recompensados ​​por su diligencia al ver el ADN en sus vasos de prueba al final del laboratorio! Intenta construir “ADN comestible” en casa: https://www.wikihow.com/Make-an-Edible-DNA-Model Translated with Google Translate

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